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截自1987年,世界各国已报道35,000多份绒毛样品(CVS)的研究结果,已知 CVS 的染色体异常频率(包括嵌合体)明显高于羊水细胞,同时观察到在同—CVS 中短期培养(直接培养)与长期培养(间接培养)的结果不一致。本文报道的 CVS短期培养与长期培养结果,其染色体嵌合体频率出现差异。本例受检孕妇年龄34岁,曾分娩过21三体婴儿,于孕9周时采取 CVS,放于 Chang 培养基内生长;羊水细胞在含有20％胎牛血清的 F-10培养基内生长。胰酶化收割细胞,制备的全部细胞均获得良好的 G 显带染色体(包括直接培养),下表列出同一个体长期和短期培养的 CVS、羊水细胞、胎血、新生儿外周血细胞的染色体分析结果:(见表)。根据产前染色体分析结果诊断为正常胎儿,继续妊娠至38周,分娩出一个表型正常的女婴,但出生后分析婴儿100个外周血淋巴细胞,94个细胞为
Since 1987, more than 35,000 samples of villus (CVS) have been reported in various countries of the world. The frequency of chromosomal abnormalities (including chimeras) of CVS is known to be significantly higher than that of amniocytes, and the short-term culture Direct training) is not consistent with the results of long-term culture (indirect culture). In this paper, short-term culture of CVS and long-term culture results, the chromosome chimera frequency differences. In this case, the age of the pregnant women tested was 34 years old and 21 trisomy babies had been delivered. CVS was taken at 9 weeks of pregnancy and placed in Chang medium for growth. Amniotic fluid cells were grown in F-10 medium containing 20% fetal bovine serum . The trypsinized cells were harvested, and all the prepared cells obtained good G-banding chromosomes (including direct culturing). The following table shows the chromosome analysis of CVS, amniotic fluid, fetal blood and neonatal peripheral blood cells of the same individual in long-term and short-term culture Results: (see table). According to the results of prenatal chromosome diagnosis of normal fetuses, pregnancy continues to 38 weeks, a normal baby girl was delivered, but after birth, 100 babies were analyzed for peripheral blood lymphocytes, 94 cells were