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The aim of this study is to explore the genomic molecular organization and genogroup of human norovirus from infected infants in Guangzhou of China.Primers were designed according to the genomic sequence of norovirus in the GenBank,and the norovirus genome was amplified by RT-PCR.The PCR- products were cloned into T vector and sequenced,and the genomic nucleotide sequences were analyzed with the programs CLUSTAL W/X,DNASTAR and RAT(Recombination Analysis Tool).The NVgz01 strain genome is 7558 bp in length and encodes three open reading frames(GenBank accession No.is DQ369797).The genomic sequences of NVgz01 were compared with those of nomvirus in GenBank, which revealed that the homology with genogroupⅡranges between 76%-90%,and genogroupⅠbe- tween 43%-44%.The ORF1 region shared 94% and 88% identity with Mc37 and Farmington strains, respectively;the capsid region(ORF2)shared 65% and 94% identity with Mc37 and Farmington strains,respectively.Phylogenetic trees were reconstructed by the neighbor-joining method.Comparative complete sequence analysis of the NVgz01 with reported human norovims genomic sequences revealed that this isolate belongs to genogmupⅡ.The ORF1 and ORF2 regions shared different identity with Mc37 and Farmington strains,suggesting NVgz01 could be a recombinant vires.
The aim of this study is to explore the genomic molecular organization and genogroup of human norovirus from infected infants in Guangzhou of China. Primers were designed according to the genomic sequence of norovirus in the GenBank, and the norovirus genome was amplified by RT-PCR. The PCR-products were cloned into T vector and sequenced, and the genomic nucleotide sequences were analyzed with the programs CLUSTAL W / X, DNASTAR and RAT (Recombination Analysis Tool). The NVgz01 strain genome is 7558 bp in length and encodes three open reading The Genomic sequences of NVgz01 were compared with those of nomvirus in GenBank, which revealed that the homology with genogroup IIrange between 76% -90%, and genogroup Ibe-tween 43% -44%. The ORF1 region shared 94% and 88% identity with Mc37 and Farmington strains, respectively; the capsid region (ORF2) shared 65% and 94% identity with Mc37 and Farmington strains, respectively. Phylogenetic trees were reconstructed by the neighbor-joining method. Comparative complete sequence analysis of the NVgz01 with reported human norovims genomic sequences revealed that this isolate isolate to to genogmup II. ORF1 and ORF2 regions shared different identities with Mc37 and Farmington, suggesting NVgz01 could be a recombinant vires.