我院自1977年开始进行了人体原位肝移植手术2例,现就移植免疫方面的部分工作,简要报导如下: 材料和方法 术前组织配型试验:包括供体和受体血型鉴定,淋巴细胞毒性试验及混合淋巴细胞培养。 血型鉴定按一般常规方法进行。 受体由静脉取血,肝素抗凝。供体于手术当天取脾血供试验用。 淋巴细胞毒性试验采用半微量法,供体脾血用密度梯度离心法分离淋巴细胞,以 In our hospital since 1977, two cases of orthotopic liver transplantation in humans were performed. Some of the work on transplantation immunity is now briefly reported as follows: Materials and Methods Preoperative Tissue Matching Assay Including Donor and Receptor Blood Type Identification, Lymphatic Cytotoxicity test and mixed lymphocyte culture. Blood type identification by conventional methods. Recipients from the venous blood, heparin anticoagulation. Donor on the day of surgery to take spleen blood for test purposes. Lymphocyte cytotoxicity test using semi-micro-method, donor spleen blood density gradient centrifugation of lymphocytes to