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目的:探讨S100A16蛋白对体重增加过程的影响,进一步研究S100A16蛋白在肥胖及肥胖相关疾病的发生发展过程中的作用。方法:正常8周龄大鼠随机分为正常饮食组(NF,n=10)和高脂饮食组(HF,n=10),建立饮食诱导的肥胖(DIO)大鼠模型,喂食14周时进行腹腔葡萄糖耐量试验(IPGTT)及胰岛素释放试验(IRT),喂食16周处死后称量皮下及内脏脂肪重量,采用HE染色方法 (hematoxylin and eosin staining,HE)观察肝脏脂肪变性程度,放射免疫分析法检测血糖、血清胰岛素、尿酸等血清生化指标,同时应用Western blot方法检测脂肪组织中S100A16及糖脂代谢相关转录因子的蛋白表达。结果:DIO组大鼠的体重、内脏脂肪明显高于正常组,血清总胆固醇和尿酸DIO组高于正常组但糖耐量和胰岛素释放低于正常组,Western blot显示DIO组大鼠脂肪和肝脏组织中S100A16、PPAR-γ、C/EBP-α的蛋白表达均高于正常组。结论 :高脂饮食可上调S100A16及相关转录因子的表达;S100A16的高表达可促进脂质生成及肥胖发生,并对机体的胰岛素敏感性产生负性影响。
OBJECTIVE: To investigate the effect of S100A16 protein on the process of weight gain, and to further investigate the role of S100A16 protein in the development of obesity and obesity-related diseases. Methods: Normal 8-week-old rats were randomly divided into normal diet group (NF, n = 10) and high fat diet group (HF, n = 10) (IPGTT) and insulin release test (IRT). The weight of subcutaneous and visceral fat was weighed after 16 weeks of sacrifice, and the degree of hepatic steatosis was observed by hematoxylin and eosin staining (HE). Radioimmunoassay The serum biochemical indexes such as blood glucose, serum insulin and uric acid were detected by Western blot. Western blot was used to detect the protein expression of S100A16 and glucose and lipid metabolism-related transcription factors in adipose tissue. Results: The body weight and visceral fat in DIO group were significantly higher than those in normal group, serum total cholesterol and uric acid DIO group were higher than those in normal group, while the glucose tolerance and insulin release were lower than those in normal group. Western blot showed that fat and liver tissue The protein expression of S100A16, PPAR-γ, C / EBP-α were higher than those in normal group. CONCLUSION: High-fat diet can up-regulate the expression of S100A16 and related transcription factors. The high expression of S100A16 can promote the formation of lipids and the incidence of obesity and negatively affect the insulin sensitivity.