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应用ＰＣＲ技术结合Ｓｏｕｔｈｅｒｎｂｌｏｔ分子杂交及免疫组合ＡＢＣ法检测８７例胃液脱落癌细胞及胃镜活检组织Ｃ－ｅｒｂＢ－２基因扩增及蛋白过度表达。结果显示：１１例（１２．６％）脱落癌细胞及１８例（２０．７％）活检组织Ｃ－ｅｒｂＢ－２基因扩增阳性；１５例（１７．２％）活检组织Ｃ－ｅｒｂＢ－２过度表达阳性；总阳性率为２５．３％（２２／８７），其中１０例（１１．５％）脱落癌细胞及活检组织基因扩增及过度表达均呈阳性反应。Ｃ－ｅｒｂＢ－２基因扩增及过度表达与胃癌进展程度、有无伴淋巴结及肝转移关系密切，结果表明Ｃ－ｅｒｂＢ－２基因检测似可作为胃癌术前估计预后的一项指标，采用上述不同方法可提高检测阳性率。
Using PCR technology combined with Southern blot hybridization and immune combination ABC method to detect 87 cases of gastric cancer exfoliated cancer cells and gastroscope biopsy C-erbB-2 gene amplification and protein overexpression. The results showed that 11 cases (12.6%) of exfoliated cancer cells and 18 cases (20.7%) of biopsy tissue were positive for C-erbB-2 gene amplification; 15 cases (17.2%) of biopsy tissue C-erbB-2 Overexpression was positive; the total positive rate was 25.3% (22/87), and 10 cases (11.5%) of exfoliated cancer cells and biopsy tissues were positive for gene amplification and overexpression. The amplification and overexpression of C-erbB-2 gene are closely related to the degree of progression of gastric cancer and presence or absence of lymph node and liver metastasis. The results show that C-erbB-2 gene detection may be used as an index to estimate preoperative prognosis of gastric cancer. Different methods can increase the positive rate of detection.