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目的研究大豆异黄酮(SI)对去卵巢大鼠抗氧化及骨形态学的影响。方法70只雌性SD大鼠按血清总胆固醇水平随机分为7组:高脂、雌激素,低、中、高剂量SI干预、假手术及正常对照组。对前5组摘除双侧卵巢后恢复1周开始干预,实验周期为12周,灌胃给药,每周称重1次,分别在去卵巢及处死时(12周)抽取尾静脉血,实验结束后取内脏及骨骼标本,分别测定血清碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶活性(GSH-Px)及骨密度等指标,共63只大鼠完成实验。结果SI干预组股骨重量高于高脂与正常对照组,干预可提高AKP、GSH-Px酶活性。高剂量干预对维持大鼠股骨密度的作用与雌激素相似。SI干预可缓解因去卵巢造成的骨丢失。结论大豆异黄酮对去卵巢大鼠的抗氧化、骨代谢酶活性及骨转化存在一定影响,适量干预能逆转因去势造成的骨密度下降。考虑到植物雌激素与哺乳动物的雌激素受体(ER)结合能力低下,采用SI在临床开展干预的剂量与远期效果尚待进一步研究。
Objective To study the effect of soybean isoflavone (SI) on antioxidation and bone morphology of ovariectomized rats. Methods Seventy female SD rats were randomly divided into 7 groups according to serum total cholesterol: high-fat, estrogen, low-, medium-, high-dose SI intervention, sham operation and normal control group. After the first 5 weeks of removal of the bilateral ovaries, the first five groups began to intervene. The experimental period was 12 weeks. The rats were administrated intragastrically and weighed once a week. Tail venous blood was collected at the time of ovariectomy and sacrifice (12 weeks). At the end of the study, visceral and skeletal specimens were taken to measure serum alkaline phosphatase (AKP), superoxide dismutase (SOD), glutathione peroxidase activity (GSH-Px), and bone mineral density. Only rats completed the experiment. Results The weight of femur in SI intervention group was higher than that in high fat and normal control group. The intervention could increase the activity of AKP and GSH-Px enzymes. High-dose interventions are similar to estrogen in maintaining femur density in rats. SI intervention can relieve bone loss caused by ovariectomy. Conclusion Soybean isoflavones have certain effects on antioxidation, bone metabolism enzyme activity and bone turnover in ovariectomized rats. Moderation intervention can reverse the decline of bone density caused by castration. Considering the low binding capacity of phytoestrogens and mammalian estrogen receptor (ER), the dose and long-term effects of SI intervention in clinical trials need further study.