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为探讨脂多糖 (lipopolysaccharide ,LPS)引起迷走传入神经活动是否可能通过白细胞介素 1(interleukin 1,IL 1)的作用 ,将Wistar大鼠随机分为LPS实验组和生理盐水对照组 ,用免疫组织化学方法检测迷走神经结状神经节c Fos及CD14的表达以及腹腔迷走神经周围Mac 1阳性巨噬细胞 (macrophage ,Mφ)。用L92 9细胞增殖法检测LPS刺激Mφ上清IL 1的生物活性。用原位杂交的方法检测迷走神经结状神经节Ⅰ型白细胞介素 1受体 (IL 1RⅠ )mRNA的表达。结果显示 ,LPS组迷走神经结状神经节神经元c Fos蛋白表达为阳性 ,而对照组迷走神经结状神经节神经元c Fos蛋白表达为阴性。LPS注射后 1h ,见腹腔迷走神经周围Mφ数量明显增多。Mφ在LPS刺激后 4 5min、1h和 2h时 ,IL 1生成明显增高。LPS组迷走神经结状神经节IL 1RⅠmRNA表达为阳性。以上结果提示 ,LPS引起迷走传入神经活动可能通过IL 1的作用
To investigate whether vagal afferent activity induced by lipopolysaccharide (LPS) might pass through the action of interleukin 1 (IL 1), Wistar rats were randomly divided into LPS experimental group and saline control group, Histochemical methods were used to detect the expression of c Fos and CD14 in the vagal nodose ganglion and macrophage (Mφ) macrophages around the celiac vagus nerve. The biological activity of LPS-stimulated Mφ supernatant IL 1 was detected by L92 9 cell proliferation assay. In situ hybridization was used to detect the expression of type Ⅰ interleukin - 1 receptor (IL 1 RI) mRNA in the vagal nodosal ganglion. The results showed that the expression of c Fos protein was positive in the neurons of vagal nodosal ganglia in LPS group, while the expression of c Fos protein in vagal ganglion neurons in the control group was negative. 1h after LPS injection, the number of Mφ around the abdominal vagal nerve was obviously increased. Mφ LPS stimulated 4 5min, 1h and 2h, IL 1 production was significantly higher. The expression of IL 1 R Ⅰ mRNA in the vagal nodose ganglion of LPS group was positive. The above results suggest that LPS-induced vagal afferent activity may be mediated by IL-1