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目的探讨杂色曲霉素(ST)对NIH小鼠的致癌作用,同时研究脱氧雪腐镰刀菌烯醇(DON)对ST致癌作用的影响。方法将180只NIH小鼠随机分为ST3μg/kg组、ST30μg/kg组、ST3μg/kg+DON1.5μg/kg组、ST30μg/kg+DON1.5μg/kg组、DON1.5μg/kg组和对照组等6组,每组30只。各实验组按要求分别灌喂不同剂量的真菌毒素,对照组灌喂同等容量的生理盐水,3次/周,共24周。实验第58周和第74周处死小鼠,观察各器官组织病变。结果对照组小鼠各器官组织均未见明显病理改变。ST和DON处理组均有部分小鼠发生肺腺癌和腺胃黏膜上皮异型增生。ST3μg/kg组、ST30μg/kg组、ST3μg/kg+DON1.5μg/kg组、ST30μg/kg+DON1.5μg/kg组和DON1.5μg/kg组肺癌的发生率分别为25.0%、41.7%、62.5%、69.2%和37.5%,腺胃黏膜上皮异型增生发生率分别为50.0%、58.3%、37.5%、53.8%和25.0%。结论经口灌喂ST和DON可诱发NIH小鼠肺癌和腺胃黏膜异型增生。ST加DON可明显提高肺癌的发生率。
Objective To investigate the carcinogenic effect of streptozotocin (ST) on NIH mice and to study the effect of deoxynivalenol (DON) on the carcinogenesis of ST. Methods 180 NIH mice were randomly divided into ST3μg / kg group, ST30μg / kg group, ST3μg / kg + DON1.5μg / kg group, ST30μg / kg + DON1.5μg / kg group and DON1.5μg / Group 6, 30 in each group. The experimental groups were fed with different doses of mycotoxins respectively according to the requirements. The control group was given the same volume of normal saline 3 times a week for 24 weeks. Mice were sacrificed on the 58th week and the 74th week respectively to observe the pathological changes of various organs. Results There was no obvious pathological changes in the organs of control mice. Some mice in ST and DON-treated groups had dysplasia of lung adenocarcinoma and glandular mucosa epithelium. The incidence of lung cancer in ST3μg / kg group, ST30μg / kg group, ST3μg / kg + DON1.5μg / kg group, ST30μg / kg + DON1.5μg / kg group and DON1.5μg / kg group were 25.0%, 41.7% 62.5%, 69.2% and 37.5% respectively. The incidence of dysplasia in glandular epithelium were 50.0%, 58.3%, 37.5%, 53.8% and 25.0% respectively. Conclusion Oral administration of ST and DON can induce dysplasia of lung cancer and glandular mucosa in NIH mice. ST plus DON can significantly improve the incidence of lung cancer.