评价乳癌和其它妇科癌肿组织中雌激素受体(ER)状态有助于预测患者对内分泌治疗的反应。现已有葡聚糖活性炭分离饱和分析法(DCC)、蔗糖密度梯度离心、凝胶电泳、凝胶过滤、高压液相层析、等电聚焦电泳及羟磷灰石法等ER生化测定方法,以及组织化学和免疫组织化学等形态学方法。其中较为常用又大家公认的参比方法是DCC法。这些方法均有不足之处。故生化学家和形态学家均在寻求更理想的ER测定方法。八十年代初期,Greene等成功地制备 Evaluating estrogen receptor (ER) status in breast and other gynecologic cancerous tissues can help predict patient response to endocrine therapy. Now available dextran activated carbon separation and saturation analysis (DCC), sucrose density gradient centrifugation, gel electrophoresis, gel filtration, high pressure liquid chromatography, isoelectric focusing electrophoresis and hydroxyapatite ER biochemical methods such as determination, As well as morphological methods such as histochemistry and immunohistochemistry. One of the more commonly used and generally accepted reference method is the DCC method. These methods have shortcomings. Therefore, biochemists and morphologists are looking for a more ideal ER assay. Early eighties, Greene, etc. successfully prepared