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目的研究IgA肾病患者外周血γδT细胞分泌TGF-β1的功能。方法用流式细胞仪分析外周血γδT细胞比率并检测血清IgA的水平,以抗TCRγδ单克隆抗体(mAb)固相法,体外选择性地扩增γδT细胞。在体外培养的条件下,以抗CD3抗体刺激48h后,用ELISA法测定培养体系中TGF-β1的水平。结果IgA肾病患者外周血γδT细胞的比例升高,并与血清IgA的水平呈正相关。用抗TCRγδmAb固相法能获得高纯度的γδT细胞。经抗CD3抗体刺激的IgA肾病患者的γδT分泌TGF-β1的量比正常对照组明显升高。结论IgA肾病患者的γδT细胞可通过大量分泌TGF-β1参与该病的发生、发展。
Objective To study the function of TGF-β1 secreted by peripheral blood of γδT cells in IgA nephropathy patients. Methods The ratio of γδT cells in peripheral blood was analyzed by flow cytometry and the level of serum IgA was detected. The γδT cells were selectively expanded in vitro by anti - TCRγδ monoclonal antibody (mAb) solid phase method. Under the condition of culture in vitro, the level of TGF-β1 in culture system was measured by ELISA after 48h stimulation with anti-CD3 antibody. Results The proportion of γδT cells in peripheral blood of patients with IgA nephropathy increased, which was positively correlated with the level of serum IgA. High-purity γδT cells can be obtained by using the anti-TCRγδmAb solid-phase method. In patients with IgA nephropathy stimulated with anti-CD3 antibody, the amount of TGF-β1 secreted by γδT was significantly higher than that of the normal control group. Conclusions γδT cells from patients with IgA nephropathy may be involved in the pathogenesis and development of the disease by secreting a large amount of TGF-β1.