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目的和方法 :X射线照射结合无血清培养诱导小鼠脾细胞凋亡 ,采用流式细胞术定量分析碱性成纤维细胞生长因子 (bFGF)对脾细胞凋亡的抑制作用 ,并采用淋巴细胞转化试验 [3H]-TdR掺入法检测bFGF对脾细胞的增殖效应。结果 :小鼠经过不同剂量X射线照射 ,脾细胞再经过不同时程无血清培养 ,都出现了典型的亚二倍体Ap峰 ,1μg/mL和 2 μg/mL的bFGF均可降低Ap峰中的细胞数 (细胞凋亡率 ) ,并能显著增强脾细胞的增殖反应能力。结论 :bFGF可抑制外周免疫器官脾细胞的凋亡 ,并促进其增殖 ,增强机体免疫力
OBJECTIVES AND METHODS: Apoptosis of mouse splenocytes was induced by X-ray combined with serum-free culture. Flow cytometry was used to quantitatively analyze the inhibitory effect of basic fibroblast growth factor (bFGF) on the apoptosis of splenocytes. Lymphocyte transformation Test [3H] -TdR incorporation assay of bFGF proliferation of spleen cells. Results: The mice were exposed to different doses of X-ray and the splenocytes were cultured in serum-free medium for different time periods. The typical sub-diploid Ap peak appeared. Both 1 μg / mL and 2 μg / mL bFGF reduced Ap peak Of the cells (apoptosis rate), and can significantly enhance the proliferative response of spleen cells. Conclusion: bFGF can inhibit the apoptosis of spleen cells in peripheral immune organs and promote its proliferation and enhance immunity