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目的：建立了测定人血浆中奥美拉唑浓度的ＨＰＬＣ测定法。方法：以普罗帕酮为内标，甲醇－醋酸－醋酸钠缓冲液（ｐＨ＝４．３）－二乙胺（５５４５０．５）为流动相，紫外检测波长为３０２ｎｍ。结果：实验表明，奥美拉唑与内标分离良好，血浆中奥美拉唑平均回收率为１０２．３５％，日内、日间ＲＳＤ均小于１０％，血浆在２０～１０００ｎｇ／ｍｌ浓度范围内线性关系良好，ｒ＝０．９９９１。本法的最低检测浓度为１０ｎｇ／ｍｌ。结论：该法稳定、可靠、灵敏度高、专一性好，适用于奥美拉唑血药浓度的测定
OBJECTIVE: To establish an HPLC method for the determination of omeprazole in human plasma. Methods: Propafenone was used as the internal standard. The mobile phase was methanol - acetic acid - sodium acetate buffer (pH = 4.3) - diethylamine (55450.5) and the UV detection wavelength was 302 nm. Results: The results showed that the omeprazole was separated from the internal standard well. The average recovery rate of omeprazole in plasma was 102.35%. The intra-day and inter-day RSD were less than 10% and the plasma concentrations were within the range of 20-1000 ng / ml Good linearity, r = 0.9991. The minimum detectable concentration of this method is 10 ng / ml. Conclusion: The method is stable, reliable, sensitive, specific and suitable for the determination of omeprazole plasma concentration