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目的探讨双酚A(BPA)暴露对高脂饲料小鼠脂肪组织炎症及Th17细胞的影响。方法高脂饲料(HFD)喂养的4周龄雄性C57BL/6小鼠,通过饮水摄入10、100和1000 nmol/L BPA,并设立正常饲料(ND)和HFD对照组。每周记录小鼠体重,BPA暴露8周和16周后处死小鼠,取附睾脂肪垫,HE染色观察脂肪组织病理学改变,酶联免疫吸附实验(EILSA)检测脂肪组织中炎症因子TNF-α的含量,流式细胞术(FCM)检测脂肪组织中Th17细胞比例,免疫组化(IHC)检测脂肪组织中IL-17的表达。结果 8周和16周时,与ND对照组相比,HFD对照组和HFD饲料BPA暴露组小鼠体重明显增加,附睾脂肪组织炎性细胞浸润增多,TNF-α含量上升,Th17细胞比例增加,IL-17表达上调;与HFD对照组相比,BPA暴露组小鼠附睾脂肪组织炎性细胞浸润增多,TNF-α含量上升,Th17细胞比例增加,IL-17表达上调,且具有剂量-效应关系;与8周时相比,16周时变化更明显。结论 BPA暴露可促进高脂饲料小鼠脂肪组织炎症、Th17细胞比例增加和IL-17表达上调;而Th17细胞可在BPA促进脂肪组织炎症中发挥作用。
Objective To investigate the effects of bisphenol A (BPA) exposure on adipose tissue inflammation and Th17 cells in high fat diet mice. Methods Four-week-old male C57BL / 6 mice fed with high fat diet (HFD) were fed with 10, 100 and 1000 nmol / L BPA by drinking water, and normal control (ND) and HFD control groups were established. The body weight of mice was recorded weekly. Mice were sacrificed at 8 weeks and 16 weeks after exposure to BPA. Epididymal fat pads were obtained. The pathological changes of adipose tissue were observed by HE staining. The levels of inflammatory cytokines TNF-α The proportion of Th17 cells in adipose tissue was detected by flow cytometry (FCM). The expression of IL-17 in adipose tissue was detected by immunohistochemistry (IHC). Results At 8 and 16 weeks, compared with ND control group, body weight of HFD control group and HFD-fed BPA-exposed mice increased significantly, inflammatory cell infiltration of epididymal adipose tissue increased, TNF-α content increased, the proportion of Th17 cells increased, Compared with HFD control group, BPA-exposed mice showed increased inflammatory cell infiltration in the epididymal adipose tissue, increased TNF-α content, increased proportion of Th17 cells and up-regulated expression of IL-17 in a dose-dependent manner ; Compared with the 8th week, the change was more obvious at 16 weeks. Conclusion BPA exposure can promote adipose tissue inflammation, increase the proportion of Th17 cells and up-regulate the expression of IL-17 in high-fat diet mice, while Th17 cells play a role in BPA-induced adipose tissue inflammation.