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目的:葛花总皂苷的提取及测定葛花生药、葛花50%甲醇浸膏、葛花总皂苷粗品中总皂苷和总黄酮的含量。方法:利用正丁醇提取,乙醚脱脂的方法富集总皂苷。利用香草醛-硫酸分光光度法测定总皂苷;采用NaNO2,Al(NO3)3,NaOH显色,分光光度法测定总黄酮。结果:葛花生药、葛花50%甲醇浸膏及葛花总皂苷粗品中总皂苷的含量分别为2.36%、4.19%、13.71%;总黄酮的含量分别为0.75%、0.96%、3.55%。结论:经过提取,葛花总皂苷和总黄酮同时得到了富集;总皂苷和总黄酮的含量测定方法简便、快速、灵敏、准确,可作为葛花质量监控的方法。
OBJECTIVE: To extract the total saponins from Pueraria lobata and determine the contents of total saponins and total flavonoids in crude peanut oil, Gehua 50% methanol extract and crude total saponin. Methods: The total saponins were enriched by n-butanol extraction and ether degreasing. The total saponins were determined by vanillin-sulfuric acid spectrophotometry. The flavonoids were determined by spectrophotometric method using NaNO2, Al (NO3) 3 and NaOH. Results: The contents of total saponins in crude peanut products, Gehua 50% methanol extract and total glucosinolate were 2.36%, 4.19%, and 13.71% respectively; the content of total flavonoids were 0.75%, 0.96%, and 3.55%, respectively. Conclusion: After extraction, the total saponins and total flavonoids of Pueraria lobata are enriched at the same time. The determination method of total saponins and total flavonoids is simple, rapid, sensitive and accurate, and can be used as a method for monitoring the quality of Pueraria lobata flowers.