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目的通过检测HPV16E6基因突变在新疆维吾尔族和汉族妇女宫颈癌组织中的分布规律,以探讨该突变与宫颈癌发生的关系。方法取汉族宫颈炎(含石蜡包埋组织及宫颈刷液基标本)125例,维吾尔族宫颈炎(含石蜡包埋组织及宫颈刷液基标本)124例;汉族石蜡包埋宫颈癌35例,维吾尔族石蜡包埋宫颈癌共109例。用以上HPV16阳性DNA模板PCR扩增HPV16E6全长基因,PCR产物直接测序,分析新疆女宫颈癌组织HPV16E6基因的突变。结果PCR检测结果显示汉族族宫颈炎组织中HPV16E6阳性率为35.71%(15/42);维吾尔族宫颈炎组织中HPV16E6阳性率为30.46%(14/46);汉族族宫颈癌组织中HPV16E6阳性率为33.33%(2/6),维吾尔族宫颈癌组织中HPV16E6阳性率为22.22%(12/54);宫颈炎与宫颈癌中HPV16E6阳性表达无统计学意义(P>0.05),对21份(上皮内低度病变1例,原位癌1例,低分化宫颈癌4例,中分化宫颈癌4例,高分化1例,轻度炎症4例,中度炎症3例,重度炎症1例,正常宫颈2例)HPV16E6扩增片段的双向测序,其中5例成功测序得到一级结构,并且序列分析表明,2例(维吾尔族宫颈癌低分化1例,中分化1例)其E6基因与原型相同,3例(汉族轻度炎症1例,汉族宫颈癌中分化1例,上皮内低度病变1例)其发生了L83V突变。结论中国新疆HPV16E6基因发生变异,其原型和变异型在该地区汉族、维吾尔族妇女宫颈癌患者组织中的分布可能与该地区宫颈癌高发存在一定的联系。
Objective To investigate the distribution of HPV16E6 gene mutation in cervical cancer of Uygur and Han women in Xinjiang to investigate the relationship between HPV16E6 mutation and cervical cancer. Methods A total of 125 Chinese women with cervicitis (including paraffin embedded tissue and cervical brush) were enrolled in this study. 124 Uygur cervicitis (including paraffin embedded tissue and cervical brush) Uygur paraffin embedding a total of 109 cases of cervical cancer. HPV16E6 full-length gene was PCR amplified with the above HPV16 positive DNA template, and the PCR product was sequenced directly to analyze the mutation of HPV16E6 gene in female cervical cancer tissues in Xinjiang. Results The positive rate of HPV16E6 in cervicitis in Han nationality was 35.71% (15/42). The positive rate of HPV16E6 in Uighur cervicitis was 30.46% (14/46). The positive rate of HPV16E6 in cervical cancer in Han nationality The positive rate of HPV16E6 in Uigur cervical cancer tissues was 22.22% (12/54). The positive rates of HPV16E6 in cervicitis and cervical cancer were not statistically significant (P> 0.05) 1 case of intraepithelial lesion, 1 case of orthotopic carcinoma, 4 cases of poorly differentiated cervical carcinoma, 4 cases of moderately differentiated cervical carcinoma, 1 case of well-differentiated carcinoma, 4 mild inflammation, 3 moderate inflammation, 1 severe inflammation, Two cases of normal cervix) bi-directional sequencing of the amplified fragment of HPV16E6, of which 5 were successfully sequenced to obtain the primary structure, and the sequence analysis showed that 2 cases (Uighur cervical cancer poorly differentiated in 1 case, moderately differentiated in 1 case) E6 gene and prototype In the same case, L83V mutation occurred in 3 cases (1 case of mild inflammation in Han nationality, 1 case of cervical cancer in Han nationality and 1 case of low intraepithelial lesion). Conclusion The variation of HPV16E6 gene in Xinjiang, China, and the distribution of its prototype and variant in cervical cancer patients of Han and Uygur women in this area may be related to the high incidence of cervical cancer in this area.