本文介绍了一种简单、快速的薄层层析法测定鲜奶与奶粉中的黄曲霉毒素M_1。黄曲霉毒素M_1用氯仿萃取,挥去氯仿后,残渣内加入四氯化碳和氯化钠—甲醇溶液,弃去四氯化碳后,再用氯仿提取,并用薄层层析法来定量。奶粉中黄曲霉毒素M_1的检出限为0.5μg/kg,用1:3硫酸显色后检出限可提高到0.3μg/kg,该法的回收率约为83%,标准偏差为11%。 This paper presents a simple and rapid TLC method for the determination of aflatoxin M_1 in milk and milk powder. Aflatoxin M_1 was extracted with chloroform. After removing chloroform, the residue was added with carbon tetrachloride and sodium chloride-methanol solution. The carbon tetrachloride was discarded and then extracted with chloroform and quantified by thin-layer chromatography. The detection limit of aflatoxin M 1 in milk powder was 0.5μg / kg. The detection limit of 1: 3 sulfuric acid was increased to 0.3μg / kg. The recovery rate was 83% and the standard deviation was 11% .