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目的研究蛇毒小分子多肽对大鼠脑缺血再灌注后脑组织肿瘤坏死因子变化的影响。方法用高效液相色谱法从五步蛇毒中提取一种小分子多肽,制造大鼠MCAO模型,静脉注射蛇毒小分子多肽后,观察TNF表达的影响。结果从五步蛇毒中提取到一种小分子多肽,分子量约为7200。此种小分子多肽能改善大鼠因缺血所致神经行为功能缺失,且随着剂量的增加效应增强;模型组大鼠缺血侧大脑皮质TNF含量显著升高,蛇毒小分子多肽处理组大鼠大脑皮质TNF含量较模型组明显下降,但仍较假手术组升高。结论蛇毒小分子多肽可能通过抑制TNF的表达,降低脑组织中TNF含量,减轻脑损伤程度,改善受损的神经功能。
Objective To study the effect of snake venom peptide on the changes of brain tumor necrosis factor after cerebral ischemia-reperfusion in rats. Methods A small molecule peptide was extracted from five-step snake venom by high performance liquid chromatography (HPLC). MCAO model was made in rats. The effect of TNF expression was observed after intravenous injection of small molecule peptides of snake venom. Results A small molecule peptide was extracted from five-step snake venom and its molecular weight was about 7200. Such small molecule peptides can improve the function of neurobehavioral deficit induced by ischemia in rats and increase the dose-dependently. The TNF level in ischemic cortex of model group is significantly increased, Compared with the model group, the content of TNF in rat cerebral cortex decreased significantly, but still higher than that in sham operation group. Conclusion The small snake venom peptide may inhibit the expression of TNF, reduce the content of TNF in brain tissue, reduce the degree of brain injury and improve the impaired neurological function.