论文部分内容阅读
AIM Initial report on the in situexamination of the mRNAexpression of transforming growth factor betas(TGFβS),TGFβ type Ⅱ receptor(TβRⅡ)and telomerase activity in theexperimental rat liver tissue during cholangiocarcinogenesis.METHODS:Rat liver cholangiocarcinogenesis was inducedby 3’-methyl 4-dimethylazobenzene(3’Me-DAB).In situhybridization was used to examine the TGFβs)and TGFβtype Ⅱ receptor(TβRⅡ)mRNA,in situ TRAP was used tocheck the telomerase activity in the tissue samples.RESULTS:There was no TGFβs,TβRⅡ mRNA expressionor telomerase activity in the control rat cholangiocytes.Theexpression of TGFβ1,TβRⅡ was increased in regenerative,hyperplastic,dysplastic chotangiocytes and cholangiocarcinoma(CC)cells.The expression of TGFβ2 mRNA was observed inonly a part of hyperplastic,dysplastic cholangiocytes.TGFβ3expression was very weak,only in hyperplastic lesion.Therewas positive telomerase activity in the regenerative,hyperplastic,dysplastic cholangiocytes,and CC cells.Stromafibroblasts of these lesions also showed positive TGFβs,TβRⅡmRNA expression and telomerase activity.CONCLUSION:There were TGFβs,TβRⅡ expression andtelomerase activity in hyperplastic,dysplastic cholangiocytes,cholangiocarcinoma cells as well as in stroma fibroblastsduring cholangiocarcinogenesis.Their expression or activityis important in cholangiocarcinogenesis andstroma formation.
AIM Initial report on the in situexamination of the mRNA expression of transforming growth factor betas (TGFβS), TGFβ type Ⅱ receptor (TβRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis. METHODS: Rat liver cholangiocarcinogenesis was induced by 3’-methyl 4- In situ TRAP was used tocheck the telomerase activity in the tissue samples. RESULTS: There was no TGFβs, TβRⅡ mRNA (TβRII) mRNA, in situ TRAP was used tocheck the telomerase activity in the tissue samples expressionor telomerase activity in the control rat cholangiocytes. Theexpression of TGFβ1, TβRII was increased in regenerative, hyperplastic, dysplastic chotangiocytes and cholangiocarcinoma (CC) cells.The expression of TGFβ2 mRNA was observed inonly a part of hyperplastic, dysplastic cholangiocytes. TGFβ3expression was very weak , only in hyperplastic lesion. Therewas positive telomerase activity in the regenerative, hyperplastic, dysplastic cholangiocytes, and C C cells. Stromafibroblasts of these lesions also showed positive TGFβs, TβRII mRNA expression and telomerase activity. CONCLUSION: There were TGFβs, TβRII expression andtelomerase activity in hyperplastic, dysplastic cholangiocytes, cholangiocarcinoma cells as well as in stroma fibroblastsduring cholangiocarcinogenesis. The expression or activity of important in cholangiocarcinogenesis andstroma formation.