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目的:观察杞菊地黄汤对实验性视网膜变性大鼠的治疗机制。方法:将生后45 d的SD大鼠分为正常组、模型对照组和杞菊地黄汤组,正常组不做处理,生后47 d中药组大鼠灌服杞菊地黄汤8.3 g.kg-1(15 mL.kg-1),模型对照组同时灌服等体积生理盐水,生后50 d ip N-甲基-N-亚硝脲(N-methyl-N-nitrosourea,MNU)40 mg.kg-1造成视网膜变性模型,正常组第55天处死,后2组大鼠分别按造模后12 h,1,2,3,5 d处死,应用免疫组织化学法检测视网膜中半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达,实时荧光定量RT-PCR法检测视网膜中Caspase-3的含量。结果:免疫组织化学和实时荧光定量RT-PCR法均显示模型对照组大鼠视网膜Caspase-3阳性表达在MNU处理后渐升高,第2天达顶峰,第5天有所下降;杞菊地黄汤组大鼠视网膜Caspase-3阳性表达第3天才达顶峰,且高峰值低于模型组。结论:杞菊地黄汤能延缓MNU ip所导致的大鼠视功能损害,其治疗机制与降低视网膜中Caspase-3的表达,从而抑制光感受器细胞的凋亡有关。
Objective: To observe the therapeutic effect of Qiju Dihuang Decoction on rats with experimental retinal degeneration. Methods: The SD rats of 45 days after birth were divided into normal group, model control group and Qiju Dihuang Decoction group. The rats in normal group were not treated at 47 days. The rats in Chinese medicine group were drenched with Qiju Dihuang Decoction 8.3 g.kg-1 (15 mL.kg-1). The model control group was given an equal volume of saline at the same time, and 50 mg N-methyl-N-nitrosourea (MNU) -1 caused retinal degeneration model, the normal group was sacrificed on the 55th day, the latter two groups were killed at 12 h, 1, 2, 3 and 5 d after modeling respectively. Immunocytochemistry was used to detect the cysteine in the retina The expression of Caspase-3 was detected by real-time fluorescent quantitative RT-PCR. Results: Immunohistochemistry and real-time fluorescence quantitative RT-PCR showed that the Caspase-3 positive expression of retina in model control group increased gradually after MNU treatment, peaked on the second day and decreased on the fifth day. Qiju Dihuang Decoction The positive expression of Caspase-3 in the retina reached its peak on the 3rd day and the peak value was lower than that of the model group. Conclusion: Qiju Dihuang Decoction can delay the impairment of visual function in rats induced by MNU ip, and its therapeutic mechanism is related to reducing the expression of Caspase-3 in the retina and thus inhibiting the apoptosis of photoreceptor cells.