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目的 :研究异黄酮Genistein对T淋巴细胞活化的影响 ,探讨将其发展为免疫干预药物的可能性。方法 :应用荧光标记的单克隆抗体和流式细胞技术 ,在全血培养体系中于 2h和 6h时间点检测分别经 10 ,5 0或 10 0 μmol L浓度Genistein预培养的 ,PHA或PDB诱导活化的T细胞的CD6 9表达百分率。结果 :培养 2h后 ,Genistein对PHA活化组的抑制作用要强于PDB活化组 ,P <0 0 5 ;培养 6h后 ,Genistein对PHA活化组的抑制作用同样强于PDB活化组 ,P <0 0 5 ,但较之 2h时间点均有所降低 ;无论PHA活化组或PDB活化组 ,Genistein对T细胞CD6 9表达率的抑制效应均随作用浓度的升高而增强。结论 :Genistein对PHA和PDB诱导活化的T细胞的CD6 9表达率均有明显的抑制作用 ,这种抑制作用存在浓度依赖关系。Genistein有潜力发展成一种免疫干预药物。
Objective: To investigate the effect of genistein on the activation of T lymphocytes and to explore the possibility of developing it as an immunological intervention drug. Methods: Fluorescently labeled monoclonal antibodies and flow cytometry were used to detect the activation of genistein preincubated with 10, 50, or 100 μmol L concentrations of PHA or PDB at 2 h and 6 h in whole blood culture system Of CD6 9 expression of T cells. Results: Genistein had stronger inhibitory effect on PHA-activated group than PDB-activated group after 2h incubation, P <0 05; after 6h incubation, Genistein also had stronger inhibitory effect on PHA-activated group than PDB-activated group, P <0.05 , But lower than that at 2h. The inhibitory effect of genistein on the CD6 9 expression rate of T cells increased with the increase of the concentration of genistein in either PHA-activated or PDB-activated groups. Conclusion: Genistein can significantly inhibit the expression of CD6 9 in PHA and PDB-stimulated T cells. The inhibitory effect of Genistein is in a concentration-dependent manner. Genistein has the potential to develop into an immunosuppressive drug.