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通过籼稻不育系及“三系”杂种的花药培养,发现花粉败育发生在单核晚期以后的雄性不育系的花粉,能正常诱导成花粉植株,并可保持雄性不育性。“三系”杂种花培时,其愈伤组织诱导率及绿苗分化串均较常规杂种及一般品种高。于较短时间内得到一些经济性状稳定而具恢复力强的“恢×恢”、“恢×保”以及杂种后代的花粉株系。在获得与原杂种较相似、产量相近
Through the anther culture of indica CMS and “three lines” hybrids, it was found that the pollen abortion occurred in the male sterile lines after the late stage of mononuclear stage and could be induced into pollen plants normally and maintain the male sterility. When the “three lines” hybrids were cultured, their callus induction rate and green shoot differentiation line were higher than conventional hybrids and common varieties. In a relatively short period of time, some pollen lines with stable economic traits, such as “Restoration × Restoration,” “Restoration × Conservation,” and hybrid offspring were obtained. In obtaining more similar with the original hybrid, yield similar