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背景:虽然对抗生素治疗无效的、伴细菌感染的中性粒细胞减少症患者被推荐输粒细胞,但受者体内白细胞抗体的存在可使这种输血无效。方法:1例25岁的男性慢性粒细胞性白血病患者在接受粒细胞输血时发生了一次肺部的输血反应,并且发现该患者对粒细胞抗原NA_2发生了免疫反应。作者对18例反复输粒细胞的慢性粒细胞性白血病患者的HLA和粒细胞抗原的免疫反应作了一项回顾性调查。结果:输粒细胞后,18例患者中有14例的血清中含有WBC抗体,有7份血清样本在淋巴细胞毒性试验、粒细胞免疫荧光法和粒细胞凝集反应中呈阳性;另有7份样本在淋巴细胞毒性试验和粒细胞免疫荧光法中有反应,但粒细胞疑集试验阴性;还有4份样本没有反应。当做粒细胞抗原的单克隆抗体的固定化试验时,3份血清样本与Fc丙种受体Ⅲ反应;3份样本有转运粒细胞抗原NB_1的58～
Background: Although neutropenic patients with bacterial infections who are not responding to antibiotic treatment are recommended to have agranulocytosis, the presence of leukocyte antibodies in the recipient may render the transfusion ineffective. METHODS: A 25-year-old male chronic myelogenous leukemia patient had a pulmonary transfusion reaction when receiving granulocyte transfusions, and the patient was found to have an immune response to the granulocyte cell antigen NA2. The authors conducted a retrospective study of the immune response to HLA and granulocyte antigen in 18 patients with relapsed chronic myelogenous leukemia. RESULTS: After transfusion of granulocytes, 14 of 18 patients had serum containing WBC antibodies, and 7 serum samples were positive in lymphocyte toxicity test, granulocyte immunofluorescence assay, and granulocyte agglutination reaction; another 7 The samples responded to lymphocyte toxicity tests and granulocyte immunofluorescence, but the granulocyte susceptibility test was negative; there were 4 samples that did not respond. For the immobilization test of monoclonal antibodies to granulocyte antigens, three serum samples reacted with Fc gamma-type receptor III; three samples had gamma-transported granulocyte antigen NB_1