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目的:观察SD大鼠乳鼠胫骨生长板软骨细胞甲状旁腺激素相关蛋白(parathyroid hormone-related protein，PTHrp)的表达情况并分析其对印度刺猬蛋白(indian hedgehog，IHH)-PTHrp信号通路的意义。方法:SD大鼠乳鼠10只，颈椎脱臼处死后取膝关节标本并制作组织学切片。应用免疫组织化学技术检测PTHrp蛋白在乳鼠胫骨生长板的表达区域，同时应用原位杂交技术检测生长板PTHrp mRNA的表达区域；应用免疫组织化学技术检测IHH-PTHrp信号通路关键因子IHH蛋白、Patched(Ptc)蛋白、PTHrp受体蛋白在胫骨生长板的表达区域。结果:乳鼠胫骨生长板软骨膜下软骨细胞、生长板前肥大区软骨细胞内均表达PTHrp蛋白及PTHrp mRNA；IHH蛋白主要在生长板前肥大区软骨细胞内表达，Ptc蛋白主要在生长板周围软骨膜细胞内表达，PTHrp受体蛋白主要在生长板增殖区软骨细胞内表达，少量在前肥大区软骨细胞内表达。结论:乳鼠胫骨生长板软骨膜下软骨细胞和前肥大区软骨细胞内均表达PTHrp蛋白；PTHrp蛋白在前肥大区软骨细胞内表达，可以补充调控胫骨生长板纵向发育的IHH-PTHrp信号通路学说。“,”Objective:To explore the expression pattern of PTHrp protein from tibial growth plate in fetal rats and to analyze its significance for the signaling pathway of Indian Hedgehog (IHH)-parathyroid hormone-related protein (PTHrp).Methods:Ten Sprague-Dawley (SD) fetal rats were sacrificed by cervical dislocation. Then the specimens of knee joint were removed and embedded in dehydrated paraffin for preparing histological sections. Immunohistochemical technique was utilized for detecting the expression of PTHrp protein and in situ hybridization for detecting the expression of PTHrp mRNA for double certification. Then immunohistochemical technique was employed for detecting the expressions of key proteins in IHH-PTHrp pathway in tibial growth plate, including IHH, Ptc and PTHrp receptor.Results:PTHrp protein and mRNA were expressed both in chondrocytes under perichondrium and prehypertrophic chondrocytes in tibial growth plate. IHH protein was predominantly expressed in preprehypertrophic chondrocytes. Ptc protein was concentrated in perichondrium around growth plate and PTHrp receptor protein in proliferative region of growth plate and a minimal amount in prehypertrophic region.Conclusions:PTHrp protein is expressed both in chondrocytes under perichondrium of fetal rats and chondrocytes of growth plate prehypertrophy. Expressed in chondrocytes of growth plate prehypertrophic region, PTHrp protein may improve the IHH-PTHrp signaling pathway theory of regulating the longitudinal development of long bone growth plate.