The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride([AMIM]Cl) is a kind of low viscous,nontoxic and recyclable ionic liquid. It was used to treat rice straw and improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows:the dosage of rice straw in [AMIM]Cl,crush mesh of rice straw,pretreatment temperature and time. After the pretreatment with a 3 %(the weight ratio of rice straw to ionic liquid) rice straw dosage in [AMIM]Cl at 110 ℃ for 1 h,the yield of reducing sugar of regenerated rice straw by 33 U/mL cellulase hydrolysis was 53.3 %,which was two times higher than that of un-treated rice straw(23.7 %) . More researches regarding straw biorefinery to bacterial cellulose are being performed in the lab and prospective results will be published in near future. The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride ([AMIM] Cl) is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice straw and improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows: the dosage of rice straw in [AMIM] Cl, crush mesh of rice straw, pretreatment temperature and time. After the pretreatment with a 3% (the weight ratio of rice straw to ionic liquid) rice straw dosage in [AMIM] Cl at 110 ° C for 1 h, the yield of reducing sugar of regenerated rice straw by 33 U / mL cellulase hydrolysis was 53.3%, which was two times higher than that of rese-straw rice (23.7%). More researches regarding straw biorefinery to bacterial cellulose are being performed in the lab and prospective results will be published in near future.