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[目的]研究锌和蔗糖与水稻根系生长发育的关系。[方法]以水稻中花11号为试验材料,分析了不同浓度的锌、蔗糖、甘露醇、锌+蔗糖和锌+甘露醇处理条件下水稻根系生长、活性氧产生和根系质子分泌的变化。[结果]在1~3mmol/L范围内随着锌浓度的增加,水稻根系总长度、数量及活性氧的产生均增加,但质子分泌水平降低。除了株高外,初生根长、初生根侧根长/数、不定根长/数和不定根侧根长/数都不同程度的受到蔗糖浓度的影响,高浓度蔗糖诱导活性氧增加,无糖或高浓度蔗糖都导致根系质子分泌能力降低。但是,在相同浓度的蔗糖处理条件下锌诱导这些指标的变化与无锌处理的明显不同。5%蔗糖和5%甘露醇对根系生长的调节有显著差异,说明该调节作用是由蔗糖信号而非渗透势引起的。[结论]该研究表明蔗糖和锌对水稻根系的生长都具有重要的调节作用。
[Objective] The research aimed to study the relationship between root growth and development of rice and zinc and sucrose. [Method] With rice Zhonghua 11 as test material, the changes of root growth, active oxygen production and root proton secretion in rice under different concentrations of zinc, sucrose, mannitol, zinc + sucrose and zinc + mannitol were analyzed. [Result] The total length of root system, the amount of rice roots and the production of reactive oxygen species increased with the increase of zinc concentration in the range of 1 ~ 3 mmol / L, but the level of proton secretion decreased. In addition to plant height, primary root length, primary root length / root number, adventitious root length / root length and number of adventitious root lateral root length / root number were affected by sucrose concentration to varying degrees, high concentration sucrose induced active oxygen increased, sugar or high sucrose All lead to reduced rootprotein secretion. However, the changes of these indices induced by zinc under the same concentration of sucrose treatment were significantly different from those of the non-zinc treatment. The regulation of root growth by 5% sucrose and 5% mannitol is significantly different, indicating that this regulation is caused by sucrose signaling rather than by osmotic potential. [Conclusion] This study shows that sucrose and zinc play an important regulatory role in the growth of rice roots.