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碱性介质中绿原酸对鲁米诺-Ag(Ⅲ)化学发光体系有显著增敏作用,在一定范围内,绿原酸的浓度与化学发光强度成正比。据此,结合流动注射技术,建立了绿原酸化学发光测定的新方法,并研究了影响化学发光强度的因素。在最优条件下测得方法的检出限为0.50μg/L,线性范围1.0—200.0μg/L。对25.0μg/L的绿原酸进行平行测定11次,其RSD为2.1%,该法已用于金银花中绿原酸含量的测定。
Chlorogenic acid in alkaline medium has significant sensitizing effect on luminol-Ag (Ⅲ) chemiluminescence system. In a certain range, the concentration of chlorogenic acid is proportional to the chemiluminescence intensity. Based on these results, a new method for the determination of chlorogenic acid by chemiluminescence was established and the factors affecting the chemiluminescence intensity were studied. The detection limit of the method under the optimal conditions was 0.50μg / L, the linear range of 1.0-200.0μg / L. The determination of 25.0μg / L of chlorogenic acid parallel determination of 11 times, the RSD was 2.1%, the method has been used for determination of chlorogenic acid in honeysuckle.