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高温用于恶性肿瘤的治疗是近年来颇受重视的工作,但高温处理不可避免地对肿瘤组织周围正常细胞也将产生影响。为了研究高温对正常细胞的影响,本文以人红细胞为实验模型,研究其细胞热损伤的机制。红细胞经不同温度处理二十分钟后,通过扫描电镜和生化分析方法探讨红细胞的形态变化,溶血作用和膜结合酶-乙酰胆碱酯酶、Na·K·Mg-ATP酶活性的变化。结果表明,在本实验条件下细胞形态的改变对高温最敏感:48℃高温处理后,绝大部分细胞变形,50℃保温使溶血显著增加,膜结合酶热变性温度则超过54℃。
The use of high temperatures for the treatment of malignant tumors is a work that has received much attention in recent years, but high temperature treatment will inevitably affect the normal cells around the tumor tissue. In order to study the effect of high temperature on normal cells, this paper uses human erythrocytes as an experimental model to study the mechanism of cell thermal damage. The erythrocytes were treated with different temperatures for twenty minutes. The erythrocyte morphological changes, hemolysis, and changes in membrane-bound enzyme-acetylcholinesterase and Na·K·Mg-ATPase activity were investigated by scanning electron microscopy and biochemical analysis. The results showed that under the experimental conditions, the change of cell morphology was most sensitive to high temperature. After high temperature treatment at 48°C, most of the cells were deformed. The incubation at 50°C increased the hemolysis significantly. The heat-denaturation temperature of membrane-bound enzyme exceeded 54°C.