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[目的]建立能同时检测甲基对硫磷、杀螟硫磷和倍硫磷的间接竞争酶联免疫分析方法(ic-ELISA)。[方法]根据ic-ELISA检测的基本程序,利用能同时识别甲基对硫磷、杀螟硫磷和倍硫磷的宽谱特异性单克隆抗体,优化相关反应的理化参数,确定最佳反应条件。[结果]建立的ic-ELISA检测方法对甲基对硫磷、杀螟硫磷和倍硫磷的灵敏度(IC_(10)值)分别为8.81、11.73、13.25μg/L;检测范围分别为12.03μg/L~0.94 mg/L、15.84μg/L~1.07 mg/L和18.92μg/L~2.79 mg/L。[结论]建立的ic-ELISA方法可以满足甲基对硫磷、杀螟硫磷和倍硫磷在果蔬及谷物中最大残留限量的检测要求。
[Objective] To establish an indirect enzyme-linked immunosorbent assay (ic-ELISA) capable of simultaneously detecting parathion, fenitrothion and fenthion. [Method] According to the basic procedure of ic-ELISA test, a broad-spectrum specific monoclonal antibody capable of simultaneously recognizing methyl parathion, fenitrothion and fenthion was used to optimize the physical and chemical parameters of the relevant reaction to determine the optimal reaction condition. [Results] The sensitivity (IC_ (10)) of the established ic-ELISA assay for methyl parathion, fenitrothion and fenthion were 8.81, 11.73 and 13.25 μg / L, respectively. The detection limits were 12.03 μg / L to 0.94 mg / L, 15.84 μg / L to 1.07 mg / L and 18.92 μg / L to 2.79 mg / L respectively. [Conclusion] The established ic-ELISA method can meet the detection requirements of the maximum residue limits of methyl parathion, fenitrothion and fenthion in fruits, vegetables and grains.