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目的优选家蝇幼虫蝇油的提取条件,研究蛆油的抗衰老作用和体外抑菌的效果。方法设计正交试验优选出家蝇幼虫蝇油的提取工艺;通过皮下注射D-半乳糖和紫外灯照射小鼠背部皮肤造成皮肤亚急性衰老模型,同时设空白、阳性对照和模型组,检测皮肤组织中丙二醛(MDA)和SOD的变化;研究蝇油的体外抗菌效果。结果蝇油的最优提取工艺为:70℃提取2.5 h,料液比为10∶1;蝇油有较好的抗衰老作用,并对大肠杆菌、金色葡萄球菌和枯草杆菌均有一定的抑菌效果。结论超声联合浸提法适合从家蝇幼虫中快速有效地提取蝇油,同时蝇油具有一定的抗衰老、抑菌作用。
Objective To optimize the extraction conditions of housefly larvae and to study the anti-aging effect of maggot oil and the antibacterial effect in vitro. Methods The orthogonal design was used to optimize the extraction process of housefly larvae. The subcutaneous acute aging model was induced by subcutaneous injection of D-galactose and UV light on the skin of the back of the mice. At the same time, blank, positive control and model groups were established. Malondialdehyde (MDA) and SOD in vitro; Results The optimal extraction technology for the flies oil was as follows: extracting at 70 ℃ for 2.5 h, the ratio of material to liquid was 10:1; the flies oil had good anti-aging effect and had certain inhibitory effect on Escherichia coli, Staphylococcus aureus and Bacillus subtilis Bacteria effect. Conclusion Ultrasound combined with the extraction method is suitable for the rapid and effective extraction of fly oil from housefly larvae, while the fly oil has certain anti-aging and antibacterial effects.