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目的:分析人早孕绒毛组织趋化因子CCL2及其受体CCR2的转录和翻译表达。方法:收集人早孕期胎盘组织30例,分离出绒毛组织,采用半定量RT-PCR、免疫组织化学法分析正常人早孕绒毛组织CCL2/CCR2的表达。结果:人早孕绒毛滋养细胞中等水平转录和翻译CCL2及CCR2。结论:CCL2/CCR2表达于母-胎界面的胎儿面绒毛组织,可能参与滋养细胞功能调控。
OBJECTIVE: To analyze the transcriptional and translational expression of chemokine CCL2 and its receptor CCR2 in human chorionic villi in early pregnancy. Methods: Totally 30 cases of placenta in early pregnancy were collected and the chorionic tissues were isolated. The expression of CCL2 / CCR2 in normal human chorionic villi was detected by semi-quantitative RT-PCR and immunohistochemistry. RESULTS: Human early gestational trophoblast cells mediate the transcription and translation of CCL2 and CCR2. Conclusion: The expression of CCL2 / CCR2 in the fetal villi of the maternal - fetal interface may be involved in the regulation of trophoblast function.