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目的研究纳米氧化锌(ZnO)颗粒对远紫外光(UVB)照射人皮肤HACaT细胞株凋亡的影响。方法实验细胞分为对照(A)组、纳米ZnO共培养(B)组、UVB照射(C)组和UVB照射ZnO共培养(D)组。采用均匀沉淀法合成纳米ZnO颗粒并运用扫描电镜(SEM)和X-射线衍射(XRD)对其进行表征。使用310nm UVB照射细胞,细胞计数试剂盒8(CCK-8)观察细胞增殖,流式细胞术观察细胞凋亡,Western blot观察细胞凋亡相关蛋白B细胞淋巴瘤-白血病2(Bcl-2)、Bcl相关X蛋白(Bax)表达。结果经SEM表征,ZnO颗粒平均粒径为(40±6)nm。纳米ZnO孵育细胞不会影响细胞的增殖,也不会导致细胞发生显著的凋亡;UVB照射细胞能抑制细胞增殖并导致细胞发生大量早期凋亡,而纳米ZnO能显著降低UVB照射细胞抑制率及凋亡率(P<0.05)。结论纳米ZnO能很好的保护HACaT细胞免受UVB的影响。
Objective To investigate the effect of ZnO nanoparticles on the apoptosis of human skin HACaT cells exposed to ultraviolet light (UVB). Methods The experimental cells were divided into control group (A), nano-ZnO co-cultured group (B), UVB irradiated group (C) and UVB irradiated ZnO co-culture group. ZnO particles were synthesized by homogeneous precipitation and characterized by SEM and XRD. The cell proliferation was observed by CCK-8 at 310 nm UVB, the apoptosis was observed by flow cytometry, and the apoptosis-related protein B-cell lymphoma-leukemia 2 (Bcl-2) Bcl-related X protein (Bax) expression. Results The average size of ZnO particles was (40 ± 6) nm by SEM. Nano-ZnO incubated cells did not affect cell proliferation, and did not lead to significant apoptosis cells; UVB irradiation cells can inhibit cell proliferation and lead to a large number of early apoptosis cells, and nano-ZnO can significantly reduce UVB irradiation inhibition rate and The apoptosis rate (P <0.05). Conclusion Nano-ZnO can protect HACaT cells from UVB effectively.