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将已知敏感性的实蝇用冷激法或麻醉法使其不能动弹,然后用各种杀虫剂的丙酮溶液以1μl的细滴定点进行处理.将在25℃下,24小时后计算出的死亡百分率和相应的用药量绘于对数概率纸上.从这些数据绘出的回归线上就能估算出LD_(50)值等等.这样就可筛选出田间有抗性的实蝇.从这种特定的剂量下存活的实蝇就是一种信号告诉我们应该作更多的测验,以确定抗性并决定其程度.
Fruit flies of known sensitivity were deprived of their animals by cold shock or anesthesia and then treated with various insecticides in acetone with 1 μl of fine titration point.After 24 hours at 25 ° C., The percentages of death and the corresponding doses were plotted on a logarithmic probability paper.On the basis of the regression lines plotted from these data, LD 50 values were estimated, etc. This allowed the screening of field-resistant fruit flies The fruit fly that survives at this particular dose is a signal telling us that more tests should be done to determine resistance and determine its extent.