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目的 毒性剂量H2 O2 可引起细胞坏死或凋亡 ,此过程是否与H2 O2 引起的核 [Ca2 +]([Ca2 +]n)变化有关未见报导。本文研究牛磺酸对H2 O2 引起的血管平滑肌细胞(VSMC) [Ca2 +]n 与胞浆 [Ca2 +]([Ca2 +]c)的变化影响。方法 应用激光共聚焦显微镜对负载Fluo 3的培养大鼠VSMC的 [Ca2 +]n 及 [Ca2 +]c 变化进行研究。结果 在0 5 %H2 O2 作用下 ,[Ca2 +]n 与 [Ca2 +]c 持续升高 ,用抗氧化细胞保护剂 牛磺酸 2 0mmol·L-1预处理细胞 ,可降低H2 O2 引起的 [Ca2 +]n升幅 (P <0 0 5 ) ,但不影响 [Ca2 +]c 升幅 (P >0 0 5 ) ,表明 [Ca2 +]n 变化与 [Ca2 +]c 变化对牛磺酸反应性存在差异。结论 VSMC的核Ca2 +调控与胞浆Ca2 +调控各自具有一定的独立性。牛磺酸对H2 O2 引起的大鼠VSMC核Ca2 +的变化具有保护作用
The purpose of toxic dose of H2O2 can cause cell necrosis or apoptosis, whether this process and H2O2-induced changes in nuclear [Ca2] ([Ca2 +] n) have not been reported. This article studies the effects of taurine on the changes of [Ca2 +] n and [Ca2 +] ([Ca2 +] c) in vascular smooth muscle cells (VSMC) induced by H2O2. Methods The changes of [Ca2 +] n and [Ca2 +] c in VSMCs cultured with Fluo 3 were studied by laser confocal microscopy. Results [Ca2 +] n and [Ca2 +] c were continuously increased in the presence of 0 5% H2O2. Pretreatment with 20 mmol·L-1 Taurine, an antioxidant cell protective agent, decreased H2O2-induced (P <0.05), but did not affect the increase of [Ca2 +] c (P> 0.05), suggesting that the change of [Ca2 +] n and the change of [Ca2 + There are differences in sex. Conclusion Both nuclear Ca2 + regulation and cytoplasmic Ca2 + regulation in VSMCs have certain independence. Taurine has a protective effect on H2O2-induced changes of nuclear Ca2 + in rat VSMCs