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目的:探讨2周耐力训练对大鼠一次急性运动骨骼肌5′-一磷酸腺苷激活的蛋白激酶(AMPK)信号的影响,分析骨骼肌AMPK信号对耐力训练的适应特点。方法:52只雄性SD大鼠分为训练和未训练两大组,训练组进行2周速度为18m/min、10%坡度、每天60min的耐力训练,结束后进行一次性大强度跑台运动(速度26m/min,10%坡度,60min);未训练组只进行一次性大强度跑台运动。两组分别于一次性运动前(安静时)、运动后即刻(0h)、1h和6h取材。使用高效液相色谱法(HPLC)测定AMP、ATP含量,放射性同位素方法测定AMPK活性。结果:与安静时相比,训练和未训练组大鼠一次性运动后即刻,腓肠肌AMP、AMP/ATP、AMPK分别增加42%、80%、80%和62%、89%、180%,均有统计学意义(P<0.01),AMP和AMP/ATP在运动后1h恢复,AMPK在运动后6h恢复;和未训练组相比,训练组一次性运动后即刻AMP和AMPK的增幅分别下降20%(P<0.05)和100%(P<0.01),但两组ATP和AMP/ATP在各时间点均无显著差异。结论:2周耐力训练削弱骨骼肌AMPK对急性运动的反应,其原因可能与AMP含量的增幅被削弱有关。
Objective: To investigate the effect of 2-week endurance training on 5’-AMP-stimulated protein kinase (AMPK) signal in primary acute exercise skeletal muscle of rats, and to analyze the adaptation characteristics of skeletal muscle AMPK signal to endurance training. Methods: Fifty-two male Sprague-Dawley rats were divided into training and untraining groups. The training group was subjected to endurance training of 18 m / min, 10% gradient and 60 min per day for two weeks. One-time intensive treadmill exercise Speed of 26m / min, 10% slope, 60min); untrained group only one-time intensive treadmill exercise. The two groups were taken before exercise (at rest), immediately after exercise (0h), 1h and 6h respectively. The contents of AMP and ATP were determined by high performance liquid chromatography (HPLC), and the activity of AMPK was determined by radioisotope method. RESULTS: AMP, AMP / AMP and AMPK increased by 42%, 80%, 80% and 62%, 89% and 180% in the gastrocnemius muscle immediately after one-time exercise in trained and untrained rats (P <0.01). AMP and AMP / ATP recovered at 1h after exercise and AMPK recovered at 6h after exercise. Compared with the untreated group, the increase of AMP and AMPK decreased 20 % (P <0.05) and 100% (P <0.01). However, there was no significant difference in ATP and AMP / ATP between the two groups at all time points. Conclusions: Two-week endurance training impaired skeletal muscle AMPK response to acute exercise, which may be related to the impaired AMP increase.