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目的:探讨射频消融(RFA)对兔肝VX2肿瘤血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响.方法:建立日本大耳白兔肝VX2肿瘤模型,随机分为3组:对照组直接处死取肝脏标本;RFA治疗(4h和24h)组于射频消融后4h和24h处死取出肝脏组织.并以正常兔肝脏组织为正常对照,通过免疫组织化学方法和逆转录聚合酶链式反应(RT-PCR)方法检测VEGF的蛋白和基因表达.结果:VX2肿瘤浸润性生长,射频后中央消融区呈现大片坏死,周围见炎性反应带,外围癌组织残留.免疫组化和RT-PCR示对照组肿瘤组织VEGF的蛋白和基因表达均高于正常组(VEGF/GAPDH基因灰度比:1.3048±0.1936vs0.8993±0.1107,P<0.05),射频消融后4h和24h表达明显下降(VEGF/GAPDH基因灰度比:0.7388±0.1503,0.8935±0.1936,均P<0.05),但不同时间段并无明显差异.结论:RFA治疗能下调肿瘤VEGF基因和蛋白水平的表达,抑制血管生成,减少血供,进而降低了复发和转移几率.
Objective: To investigate the effect of radiofrequency ablation (RFA) on the expression of vascular endothelial growth factor (VEGF) in rabbit hepatic VX2 tumor.Methods: To establish the model of liver VX2 tumor in Japanese white rabbits, and randomly divided into three groups: control group (4 h and 24 h) were sacrificed at 4h and 24h after radiofrequency ablation and the normal liver tissue of the rabbits was taken as the normal control.Immunohistochemistry and reverse transcription polymerase chain reaction RT-PCR) was used to detect the protein and gene expression of VEGF.Results: The VX2 tumor infiltratively grew in the central ablation area after radiofrequency and showed necrosis around the inflammatory reaction zone and peripheral cancer tissue.Immunohistochemistry and RT-PCR showed The protein and gene expression of VEGF in control group were significantly higher than those in normal group (the gray ratio of VEGF / GAPDH gene was 1.3048 ± 0.1936vs0.8993 ± 0.1107, P <0.05) GAPDH gene gray scale ratio: 0.7388 ± 0.1503,0.8935 ± 0.1936, all P <0.05), but there was no significant difference at different time points.Conclusion: RFA can decrease the expression of VEGF gene and protein, inhibit angiogenesis and decrease blood Supplied, then reduced And metastatic chance.