本研究在初步实现水稻原生质体培养的程序化后,选用普通栽培稻P339和特种稻苏御糯选的原生质体为融合亲本,利用碘乙酸(IA)和罗丹明-6G(R-6G)这两个代谢互补抑制剂钝化处理亲本原生质体,确定了合适的抑制条件。P339用0.25mmol/L IA,苏御糯选用50μg/ml R-6G分别经30min钝化处理,通过PEG和高Ca~(2+)、高pH法诱导融合,异源融合体具有代谢互补效应,经培养得到愈伤组织17块,并进一步分化获得不同形态的再生植株12棵。移栽存活的再生植株成熟后可育,通过对这些植株的形态以及酯酶和过氧化物酶同工酶电泳的分析表明是融合后的体细胞杂种植株。 In this study, protoplasts from common cultivated rice P339 and special rice Suyu Nuo were selected as fusion parents after the preliminary realization of protoplast prototyping of rice, using iodine-acetic acid (IA) and rhodamine-6G (R-6G) Two metabolites of complementary inhibitors passivated the parental protoplasts to determine the appropriate inhibition conditions. P339 was treated with 0.25mmol / L IA and Suyu Nuo 50μg / ml R-6G for 30min respectively. The fusion was induced by PEG and high Ca 2+, high pH. The heterologous fusion had the effect of metabolite complement 17 calluses were obtained after culturing, and further 12 regenerated plants of different morphologies were obtained. The transplanted surviving regenerated plants are fertile after maturity, and analysis of the morphology and esterase and peroxidase isoenzyme electrophoresis of these plants shows that they are hybrid somatic hybrid plants.