论文部分内容阅读
油菜叶片经油菜菌核病菌 （ Ｓｃｌｅｒｏｔｉｎｉａ ｓｃｌｅｒｏｔｉｏｒｕｍ ） 或水溶性壳聚糖处理后， 几丁质酶活性明显提高， 分别在处理后 １ ｄ 和 ２ ｄ 达活性高峰， 比对照分别高５ 倍和２ 倍左右。被激发提高的几丁质酶表现为内切酶活性， 细胞间的几丁质酶比活力显著高于细胞内的酶比活力。菌核病菌侵染后的油菜叶片提取液经６０％ 饱和度的硫酸铵沉淀，再通过再生几丁质柱亲和层析得到了纯化的几丁质酶， 经 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 显示单一的谱带， 其相对分子质量为 ３２ ０００。纯化的油菜叶片几丁质酶能降解油菜菌核病菌菌丝细胞壁， 在马铃薯葡萄糖琼脂培养基上可抑制菌丝生长。
After treated with Sclerotinia sclerotiorum or water-soluble chitosan, the chitinase activity of rapeseed leaves significantly increased, reaching the peak of activity at 1 and 2 days after treatment, respectively, which were 5 times and 2 times higher than that of the control about. Chitinase was enhanced by endonuclease activity. The specific activity of chitinase between cells was significantly higher than that of intracellular enzyme. Sclerotinia sclerotiorum rape leaf extract after precipitation by 60% saturation of ammonium sulfate precipitation, and then regenerated by chitin column affinity chromatography purified chitinase obtained by S D S-P A G E showed a single Of the band, the relative molecular mass of 32 000. The purified rapeseed chitinase can degrade the mycelial cell wall of rape Sclerotinia mycelium and inhibit mycelial growth on potato dextrose agar.