尿激酶是一种纤溶酶原激活剂,由肾脏合成并释放到尿中。临床上用于治疗血栓,商品酶是从大量人尿中提取,纯化方法复杂,不易得到纯品。抗尿激酶单克隆抗体亲和柱有可能简单而有效地纯化尿激酶,为此我们采用杂交瘤技术制备了七个分泌抗尿激酶单克隆抗体的杂交瘤。它们分泌的单克隆抗体分别命名为S_(31)、S_(26)、N_(14)、N_(17-2)、N_(30)、N_(34)和N_(36)。 Urokinase is a plasminogen activator synthesized by the kidneys and released into the urine. Clinically for the treatment of thrombosis, commercial enzyme is extracted from a large number of human urine, purification methods are complex, not easy to get pure. It is possible to purify urokinase easily and effectively by anti-urokinase monoclonal antibody affinity column. For this reason, we prepared seven hybridomas secreting anti-urokinase monoclonal antibodies by hybridoma technology. Monoclonal antibodies secreted by them were named as S 31, S 26, N 14, N 17-2, N 30, N 34 and N 36, respectively.