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目的 研究17种抗生素对16种标准β-内酰胺酶的稳定性。方法 采用紫外分光光度法。结果 第三代头孢菌素和氨曲南(除头孢哌酮外)均对β-内酰胺酶稳定,其中头孢三嗪的酶稳定性最好,相对水解率均不超过30%。结论 第二代头孢菌素头孢呋新的相对水解率为6%~65%。第一代头孢菌素对酶的稳定性不如第二代头孢菌素,其中头孢硫脒对酶的稳定性尤差,OXA-2、SHV-4、D31对它的相对水解率分别高达523%、912%和359%。青霉素类的酶稳定性较差,大多数相对水解率在100%以上。
Objective To study the stability of 16 standard β-lactamases by 17 antibiotics. Methods UV spectrophotometry. Results The third generation cephalosporins and aztreonam (except cefoperazone) were stable to β-lactamase, of which the best ceftriaxone enzyme stability, the relative hydrolysis rate of not more than 30%. Conclusion The relative hydrolysis rate of second-generation cephalosporin cefuroxime 6% to 65%. The first-generation cephalosporins on the stability of the enzyme as second-generation cephalosporins, of which cefathiamidine stability of the enzyme especially poor, OXA-2, SHV-4, D31 its relative hydrolysis rate as high as 523% , 912% and 359% respectively. Penicillins have poorer enzyme stability, with most relative hydrolysis rates above 100%.