论文部分内容阅读
证实了以转移于硝酸纤维膜(NC,paper)的标准蛋白为参照标准(Marker)用SDS-PAGE结合电洗脱技术,分离纯化靶蛋白的可行性。用该技术分离井纯化了旋毛虫肌肉期幼虫分泌排泄物(TsL1-ES)中具免疫原性的抗原组份-46~58kD蛋白,获得近2mg具有明显免疫反应性的高纯度的靶蛋白组份。为进一步研究该特异性抗原组份的诊断价值和保护性免疫作用提供了先决条件。
The feasibility of isolating and purifying the target protein by using SDS-PAGE combined with electroelution technique with a standard protein transferred to a nitrocellulose membrane (NC, paper) as a reference standard was confirmed. This technique was used to separate and purify the immunogenic antigen component-46 to 58kD protein in the secretion and excretion (TsL1-ES) of Trichinella spiralis muscle muscle, and to obtain nearly 2mg highly purified target protein group with obvious immunoreactivity Copies Which provided the preconditions for further research on the diagnostic value and protective immunity of this specific antigenic component.