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以红肉火龙果的气生根为试材,研究气生根长度、采样时间、预处理方法、解离时间和染色方法等对红肉火龙果气生根根尖细胞染色体制片效果的影响,以优化的制片技术制片并进行核型分析。结果表明:10:30时段取长度5mm以下的幼嫩气生根,用0.1%秋水仙素常温预处理2h,应用双低渗法使染色体分散,常温下1mol/L HCl解离50min或60℃下解离20min,20%改良苯酚-品红染色液染色20min,可以得到最佳的制片效果,用中性树脂制成永久装片。染色体核型分析表明,红肉火龙果的染色体数目为11对,最长染色体/最短染色体为1.63,核型公式为2n=2x=22=22m,核型不对称系数为54.29%,核型属1A类型。
The effects of air root length, sampling time, pretreatment method, dissociation time and dyeing method on the chromosome preparation of root tip cells of red meat dragon fruit were studied in order to optimize The production technology and production of karyotyping. The results showed that the young shoots with the length of 5 mm or less were pretreated with 0.1% colchicine at room temperature for 2 h at 10:30, and the chromosomes were dispersed by double hypotonic method. The dissociation of 1 mol / L HCl at room temperature for 50 min or 60 ° C Dissociation 20min, 20% modified phenol - magenta stain staining 20min, you can get the best filming effect, made of a neutral resin permanent loading. Chromosome karyotype analysis showed that the number of chromosomes in red meat dragon fruit was 11 pairs, the longest chromosome / shortest chromosome was 1.63, the karyotype formula was 2n = 2x = 22 = 22m, the karyotype asymmetry coefficient was 54.29% 1A type.