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细胞凋亡抑制基因bcl-2在髓系造血细胞中的表达与细胞分化程度有关。本研究证实全反式维甲酸(ATRA)可诱导HL-60细胞凋亡。流式细胞仪分析表明,在HL-60细胞分化凋亡过程中bcl-2表达逐渐下降,凋亡细胞bcl-2表达水平较低,而未凋亡细胞bcl-2仍维持在高水平。上述结果提示:经ATRA诱导分化成熟的HL-60细胞与正常粒细胞相似,最终走向凋亡;bcl-2表达降低可能对终末分化细胞凋亡有易化作用;未凋亡细胞bcl-2高表达可能是肿瘤细胞耐药和白血病复发的重要因素。
The expression of apoptosis-suppressing gene bcl-2 in myeloid hematopoietic cells is related to the degree of cell differentiation. This study confirmed that all-trans retinoic acid (ATRA) can induce HL-60 cell apoptosis. Flow cytometry analysis showed that the expression of bcl-2 in HL-60 cells gradually decreased, the expression of bcl-2 in apoptotic cells was lower, and the level of bcl-2 in non-apoptotic cells remained high. The above results suggest that HL-60 cells induced by ATRA are similar to those of normal granulocytes and eventually apoptosis; the decrease of bcl-2 expression may facilitate the differentiation of terminally differentiated cells; the expression of bcl-2 High expression may be an important factor in tumor cell resistance and relapse of leukemia.