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目的:探讨芦荟大黄素(AE)对人膀胱癌细胞株T24细胞增殖和凋亡的影响及其可能的机制。方法:采用甲基噻唑(MTT)比色法测定AE对体外培养的T24细胞的抑制率,并用作图法计算半数有效抑制浓度(IC5)0;光学显微镜下观察AE作用前后细胞形态学变化;流式细胞技术检测细胞增殖周期及凋亡率的变化;逆转录-聚合酶链反应(RT-PCR)法检测mRNA表达。结果:MTT法测定显示,在5～160μg/mL浓度范围内,AE对膀胱癌T24细胞生长有抑制作用,并呈量-效关系,IC50为31.55μg/mL;光学显微镜下药物IC50剂量(32μg/mL)作用T24细胞后呈现明显的死亡形态变化;流式细胞仪检测显示AE可阻滞T24细胞进入G2/M期;Annexin V-FITC/PI双染法显示早期凋亡细胞数明显增加;R T-PCR法显示myc基因表达下降。结论:AE能抑制人膀胱癌T24细胞生长,并阻断细胞周期和诱导细胞凋亡。其机制可能与myc基因表达下调有关。
Objective: To investigate the effect of aloe-emodin (AE) on proliferation and apoptosis of human bladder cancer cell line T24 and its possible mechanism. Methods: MTT assay was used to determine the inhibition rate of AE on T24 cells cultured in vitro, and used to calculate the half effective inhibitory concentration (IC50) 0; Morphological changes before and after AE were observed under light microscope; Flow cytometry was used to detect cell proliferation cycle and apoptosis rate; RT-PCR was used to detect mRNA expression. Results: The results of MTT assay showed that AE could inhibit the growth of bladder cancer T24 cells in the concentration range of 5 ~ 160μg / mL with a dose-response relationship (IC50 of 31.55μg / mL); IC50 of IC50 (32μg / mL) showed obvious morphological changes of death after T24 cells. Flow cytometry showed that AE could block T24 cells entering G2 / M phase; Annexin V-FITC / PI double staining showed that the number of apoptotic cells increased significantly; R T-PCR showed decreased myc gene expression. Conclusion: AE can inhibit the growth of human bladder cancer T24 cells and block the cell cycle and induce apoptosis. The mechanism may be related to the down-regulation of myc gene expression.