目的　构建含单纯疱疹病毒 胸苷激酶 (HSV tk)和人白介素 2 (hIL 2 )基因的痘苗病毒真核表达载体 pMJ6 0 1,为进一步实施胃癌的基因治疗作必要的准备。 方法　利用目的基因与载体的连接、感受态细胞的制备及转化、质粒抽提、琼脂糖凝胶电泳、酶切、DNA序列分析等多种基因工程技术 ,将纯化回收的HSV tk和hIL 2分别与 pMJ6 0 1进行连接、转化并鉴定。 结果　分别用BamHⅠ HindⅢ和SalⅠ BamHⅠ酶切位点 ,将HSV tk与hIL 2DNA成功地克隆到pMJ6 0 1真核表达载体上。结论　重组HSV tk +hIL 2基因 pMJ6 0 1的构建 ,为进一步研究胃癌的基因治疗打下坚实的基础。 Objective To construct an eukaryotic expression vector pMJ106 containing vaccinia virus herpes simplex virus (HSV tk) and human interleukin 2 (hIL 2) genes for the further implementation of gene therapy for gastric cancer. METHODS: The HSV tk and hIL 2 were purified and purified by various gene engineering techniques such as ligation of the target gene and vector, preparation and transformation of competent cells, plasmid extraction, agarose gel electrophoresis, enzyme digestion, and DNA sequence analysis. The pMJ610 was ligated, transformed and identified. Results HSV tk and hIL 2 DNA were successfully cloned into pMJ610 eukaryotic expression vector using BamHI HindIII and SalI BamHI restriction sites, respectively. Conclusion The construction of recombinant HSV tk + hIL 2 gene pMJ61 has laid a solid foundation for further research on gene therapy of gastric cancer.