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目的在证明核酸疫苗pcDNA3．1(+)．Sj14-3．3具有部分抗血吸虫感染的基础上,联合使用CpG和pcDNA3．1(+)．mIL-12,观察此二种佐剂在攻击感染小鼠的免疫效果及其抗血吸虫感染的保护机制。方法分别用pcDNA3．1(+)．sj14-3．3+pcDNA3．1(+)．mIL-12、pcDNA3．1(+)．Sj14-3．3 +CpG、pcDNA3．1(+)．mIL-12和CpG免疫小鼠。攻击感染后6w计数成虫负荷和肝虫卵数;检测免疫后0w、6w和12w小鼠血清总IgG、IgG1和IgG2a水平;检测小鼠脾细胞培养上清中IFN-γ和IL-4;流式细胞术检测免疫鼠脾细胞中CD4+和CD8+T细胞的比率。结果pcDNA3．1(+)-Sj14-3．3+pcDNA3．1 (+)-mIL-12和pcDNA3．1(+)-Sj14-3．3+CpG免疫小鼠的减虫率分别为41．2％和28．7％;减卵率分别为52．6％和41．2％。单独使用pcDNA3．1(+)-mIL-12和CpG也有一定的免疫保护作用。保护性免疫主要通过诱导宿主产生CTL、TH1型和体液免疫应答。结论pcDNA3．1(+)-mIL-12和CpG具有较强的增强核酸疫苗pcDNA3．1(+)-Sj14-3．3抗血吸虫攻击感染作用。
The purpose of the proof of DNA vaccine pcDNA3.1 (+). Sj14-3.3 has partial anti-schistosome infection based on the combination of CpG and pcDNA3.1 (+). mIL-12, to observe the immune effect of the two adjuvants in challenge-infected mice and the protective mechanism of anti-schistosomiasis infection. Methods Using pcDNA3.1 (+). sj14-3.3 + pcDNA3.1 (+). mIL-12, pcDNA3.1 (+). Sj14-3.3 + CpG, pcDNA3.1 (+). MIL-12 and CpG immunized mice. The load of adult worms and the number of hepatic eggs were counted 6 h after the challenge. The levels of total IgG, IgG1 and IgG2a in serum were detected at 0w, 6w and 12w after immunization. The levels of IFN-γ and IL-4 The percentage of CD4 + and CD8 + T cells in splenocytes of immunized mice was detected by cytometry. Results The worm reduction rates of mice immunized with pcDNA3.1 (+) - Sj14-3.3 + pcDNA3.1 (+) - mIL-12 and pcDNA3.1 (+) - Sj14-3.3 + CpG were 41. 2% and 28.7% respectively; the oviposition rates were 52.6% and 41.2% respectively. PcDNA3.1 (+) - mIL-12 alone and CpG also had some immunoprotective effects. Protective immunity mainly produces CTL, TH1 type and humoral immune responses by inducing the host. Conclusion pcDNA3.1 (+) - mIL-12 and CpG have stronger anti-schistosoma infection effects of enhanced DNA vaccine pcDNA3.1 (+) - Sj14-3.3.