论文部分内容阅读
盐酸麻黃素注射液及片剂的含量測定有用乙醚提取后的中和法,水蒸汽蒸溜法;不經提取的銀量法,非水滴定法等数种。前二法均需經过抽提手續,且水蒸汽蒸溜較为費时,故存在一定缺点。由于片剂輔料的影响,銀量法及非水滴定法亦非理想。文献报道的盐酸麻黄素此色法很多。如在硷性条件下,加过氧化氢在沸水浴上加热得一紫色溶液。在苯液中与2、4、6三硝基氯苯作用,在75～77℃水浴上加热20分钟,放冷,再在400毫微米測定光密度。Wachsmuth采用1滴1%茚三酮溶液和1滴1%Na_2GO_3溶液与盐酸麻黃素溶液加热得一紫色溶液,轉溶于戊醇中,在550毫微米测定光密度。本文利用Wachsmuth的茚三酮反应,进行了試驗研究,找出了一个不需要轉溶于戊醇中的比色测定方法,应用于本品注射液与片剂的测定,結果尚称滿意。
Determination of ephedrine hydrochloride injection and tablet neutralization after extraction of useful ether, steam distillation; without extraction of silver, non-water titration and other species. The first two methods are subject to extraction procedures, and steaming more time-consuming, so there are some shortcomings. Due to the influence of tablet accessories, the silver method and the non-water titration method are also not ideal. Reported in the literature of ephedrine hydrochloride lot color method. For example, under alkaline conditions, hydrogen peroxide is heated in a boiling water bath to obtain a purple solution. In benzene with 2,4,6 trinitrochlorobenzene role in 75 ~ 77 ° C water bath for 20 minutes, let cool, and then measured at 400 nm optical density. Wachsmuth was heated with 1 drop of 1% ninhydrin solution and 1 drop of 1% Na 2 GO 3 solution with ephedrine hydrochloride solution to obtain a purple solution, dissolved in amyl alcohol and measured for optical density at 550 nm. In this paper, Wachsmuth’s ninhydrin reaction was used to conduct a pilot study to identify a colorimetric assay that does not require conversion to pentanol. The results are still satisfactory.