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目的 观察 p SVPo Mcat微基因修饰雪旺细胞 (SC)在鼠脊髓内存活及基因表达 .方法 实验动物分为 3组 ,A组 p SVPo Mcat微基因修饰 SC移植组 ;B组高纯化 SC移植组 ;C组为脊髓损伤 (SCI)对照组 .术后动物分组存活 2 ,4,8及 12 wk(每组每次 2 0只 ) ,取移植区脊髓切片 ,进行 S- 10 0蛋白 ,髓鞘碱性蛋白 (MBP)免疫细胞化学染色及地高辛 (DIG)标记的 h MBE2 c DNA探针的原位杂交 (ISH)测定 .结果 术后 2 ,4,8及 12 wk,A组的 S- 10 0和 MBP染色细胞以及ISH细胞分别为 ,(82 .3± 6 .0 7) % / (77.8± 12 .3) % / (6 7.8±8.6 ) % ;(81.2± 5 .2 ) % / (76 .3± 11.8) % / (6 8.4± 8.3) % ;(80 .7± 5 .6 ) % / (75 .4± 11.6 ) % / (6 6 .4± 8.2 ) % ;(81.6±6 .2 ) % / (75 .8± 9.5 ) % / (6 3.3± 8.0 2 ) % (P>0 .0 5 ) .其中ISH阳性细胞可见髓鞘形成 ,B组术后 2 ,4,8及 12 wk的 S-10 0染色的细胞为 ,(6 6 .3± 6 .8) % ,(4 0 .2± 11.2 ) % ,(10 .8± 2 .6 ) % ,(3.2± 0 .6 ) % (P<0 .0 0 1) .未发现 MBP染色细胞以及 ISH细胞、C组未发现任何阳性细胞 .结论 p SVPo Mcat微基因修饰 SC在鼠损伤脊髓内能长期存活并表达外源基因 ,且有助于受伤脊髓的髓鞘形成 .它们为脊髓损伤的基因治疗提供了可行途径
Objective To observe the survival and gene expression of SVPo Mcat micro-gene modified Schwann cells (SCs) in the spinal cord of rats.Methods The experimental animals were divided into three groups: group A, SVpO Mcat micro-gene modified SC transplantation; group B, Group C was SCI control group, and the animals survived for 2, 4, 8 and 12 wk (20 in each group) after operation, and the spinal cord slices of the transplantation area were taken for S-10 protein, myelin sheath Immunocytochemical staining of basic protein (MBP) and in situ hybridization (ISH) of digoxin (dIG) labeled h MBE2c DNA probe were performed.Results After 2, 4, 8 and 12 wk, S (82.3 ± 6.07)% / (77.8 ± 12.3)% / (6 7.8 ± 8.6)%, (81.2 ± 5.2)% and MBP-stained cells and ISH cells / (76.3 ± 11.8)% / (6 8.4 ± 8.3)%; (80.7 ± 5 .6)% / (75.4 ± 11.6)% / (6.6 ± 8.2)%; ± 6 .2% / 75.8 ± 9.5% / 6 3.3 ± 8.0 2% (P> 0.05), among which ISH positive cells showed myelination, while in group B, 2, 4, S-10 0-stained cells at 8 and 12 weeks were (6 6 .3 ± 6 .8)%, (402 ± 11.2)%, (10.8 ± 2 .6)%, (3.2 ± 0 .6)% (P <0. 0 0 1). Not found MBP-stained cells and ISH cells, no positive cells were found in group C. CONCLUSIONS p SVPo Mcat microgene modified SC can survive long-term and express foreign genes in injured spinal cord and contribute to myelination of injured spinal cord. Gene therapy of spinal cord injury provides a viable approach