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从 1999年 10月至 2 0 0 1年 3月对导致栉孔扇贝 (Chlamysfarreri)大规模死亡的可疑病原进行了系统调查 ,在栉孔扇贝体内发现 1种细胞内寄生原核生物。根据该原核生物超微形态结构及所形成的包涵体形态特征及染色性质分析 ,初步确定为类立克次体 (Rickettsia likeorganisms ,RLO)。该RLO大小为 (3.6 2 3± 1.4 35 ) μm× (1.343± 0 .32 6 ) μm (n =4 5 ) ,主要寄生在栉孔扇贝的鳃、消化腺的上皮组织中。其感染率和感染强度与水温及栉孔扇贝死亡率呈负相关关系。人工感染试验证明 ,RLO可引起栉孔扇贝感染 ,但不形成大面积明显的组织病理变化。本研究表明 ,RLO对栉孔扇贝不具明显的致病性 ,不是导致栉孔扇贝大规模死亡的主要原因。
From October 1999 to March 2001, a systematic investigation into the suspicious pathogen causing large-scale death of Chlamys farreri was carried out, and one intracellular parasitic prokaryotic organism was found in Chlamys farreri. According to the ultrastructure of the prokaryotes and the morphological characteristics of the formed inclusion bodies and the analysis of the staining properties, Rickettsia like organisms (RLO) were initially identified. The size of the RLO was (3.6 2 3 ± 1.4 35) μm × (1.343 ± 0.32 6) μm (n = 45), which was mainly parasitized in the gills and digestive glands of the scallop. The infection rate and infection intensity and water temperature and the mortality of scallop was negatively correlated. Artificial infection test proved that RLO can cause chlamydia infection, but does not form a large area of significant histopathological changes. This study shows that RLO has no obvious pathogenicity on the chlamys farreri and is not the main cause of the large-scale death of the chlamys farreri.