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采用保温交换法制备阿克拉霉素（ＡＣＭ－Ａ）低密度脂蛋白（ＬＤＬ）复合物，荧光光度法测定细胞内ＡＣＭ－Ａ含量，研究宫颈癌Ｈｅｌａ细胞对ＡＣＭ－Ａ－ＬＤＬ复合物及游离ＡＣＭ－Ａ的摄取、细胞对复合物的摄取与ＬＤＬ受体（ＬＤＬＲ）活性的关系及该复合物对Ｈｅｌａ细胞ＤＮＡ及ＲＮＡ合成的抑制作用。结果：①在相同作用时间下，宫颈癌Ｈｅｌａ细胞对ＡＣＭ－Ａ－ＬＤＬ复合物的摄取显著高于游离ＡＣＭ－Ａ的摄取；②细胞对复合物的摄取受ＬＤＬＲ饱和度的限制；③ＡＣＭ－Ａ－ＬＤＬ复合物对宫颈癌细胞ＤＮＡ及ＲＮＡ的合成具有较强地抑制作用。
ACM-A low-density lipoprotein (LDL) complex was prepared by heat exchange method. The content of ACM-A in cells was determined by fluorescence spectrophotometry, and the complex of ACM-A-LDL and its free from Hela cells were studied. The uptake of ACM-A, the uptake of complexes by cells and the activity of LDL receptor (LDLR), and the inhibitory effect of the complex on HeLa DNA and RNA synthesis. RESULTS: 1 Under the same action time, the uptake of ACM-A-LDL complex in cervical cancer Hela cells was significantly higher than that of free ACM-A; 2 The uptake of complex by cells was limited by the saturation of LDLR; 3ACM-A The LDL complex has a strong inhibitory effect on the synthesis of DNA and RNA in cervical cancer cells.