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从百合中提取皂苷 ,在Fenton反应产生羟自由基的稳定体系中 ,以溴邻苯三酚红为显色剂 ,用分光光度法测定吸光度的变化值 ,研究百合皂苷在此体系中清除羟自由基的作用。通过常见抗氧化剂对羟自由基清除作用的对照实验 ,表明 :百合皂苷作为天然产物对羟自由基有较好的清除效果 ,本法操作简便 ,稳定性好 ,测定快速 ,为开发百合复方保健品提供理论依据
The saponin was extracted from Lily, and the change of absorbance was measured by spectrophotometry with bromopyrogallol red as a color reagent in a stable system for the hydroxyl radical generated by the Fenton reaction. The Lily saponin scavenging hydroxyl free in this system was studied. The role of the base. The control experiment of hydroxyl radical scavenging effect by common antioxidants showed that lilidum saponin as a natural product has a good scavenging effect on hydroxyl radicals. This method is easy to operate, has good stability, and is rapid in determination. It is a lily compound health product development. Provide theoretical basis